Applications of Confocal Laser Scanning Microscopy in Dentistry. Study of the changes of the post-extraction sites
نویسندگان
چکیده
The Confocal Laser Scanning Microscope (CLSM) is an optical microscope that includes a laser light as a light source and an electronic system which helps on image processing. It obtains high-resolution and extremely thin optical image sections, removing the interference caused by the light arriving from the different optical fields across the thickness of the sample, and focusing on a single plane (confocal). Therefore, since the images obtained are digital images, unsuspected magnifications for optical microscopy can be obtained. The CLSM works as follows: it removes the veil that is generated in the regions that are placed outside the focus plane in a normal optical microscope image. The incident light on the sample passes through a small hole or slot (pinhole) and focuses on the image plane of a high numerical aperture objective, so that the light reflected by the point which is placed in the focal plane of the objective returns to it and is refocused and transmitted by a pinhole without any loss. By contrast, the light scattered or emitted by the points outside the image plane is dimmed or completely blocked. Thus, an image of high contrast and definition of a point on the focal plane is obtained without being affected by the regions which are out of focus. As the apertures of both the lighting and the return of the image have a common focus, this kind of microscopes have been called Confocal Microscope. In summary, Confocal Microscopy is based on an improved relationship between the signal and image noise.
منابع مشابه
CONFOCAL LASER SCANNING MICROSCOPY (CLSM) FOR THE STUDY OF THE MORPHOLOGICAL CHANGES OF THE POST-EXTRACTION SITES Brief title: CLSM and post-extraction changes
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